Which of the little women in Little Women (1933) did Katharine Hepburn play? July 6, 2020 by Pamela Landy Katharine Hepburn played Jo March, the eldest of the little women in Little Women (1933). Related Answers What is Katharine Hepburn’s origin? What was Katharine Hepburn’s film debut? In what movie did Audrey Hepburn play a cigarette girl? Who played the three women in Robert Altman’s Three Women (1977)? What is Audrey Hepburn’s national origin? What is Holly Golightly’s (Audrey Hepburn’s) real name in Breakfast at Tiffany’s (1961)?